Monkeypox (mpox) virus (MPXV) load in various clinical samples. MPXV revealed various tissue tropisms. Although optimal clinical specimens for laboratory analyses depend on the disease stages or infectious routes, typical skin lesions are frequently used because skin lesions occur frequently, can be easily harvested, and have high viral loads in the lesions. In addition to skin lesions, MPXV DNA was detected in saliva or oral mucosal swab, rectal swab, nasopharyngeal swab, semen, urine, and feces. The Ct (cycle threshold) is defined as the number of cycles required for the fluorescent signal to cross the threshold or background (adapted from the article of Peiró-Mestres et al. Euro Surveill 2022;27:2200503 [
48]).